I've always gotten the best results by using "Color Control" rather than "No Color Correction" in the Epson Twain Driver (Ver 3.45A). I have an Epson Perfection 1250 scanner and found that I have to play around with the black and white sliders in the Histogram Adjustment to get the best results (see first snap below). I'm not at the computer that I used to use, but I think the values were somewhere around 10-15 for black and 240-245 for white. It's pretty much trial and error, scan the IT8, bring it into PP, set the corners, let it chug away, then evaluate the resultant histogram for low- and high-end clipping (try to minimize the heights of the red bars; second snap below). Once the optimal values have been determined, I save the setup in the Twain interface so that I can recall those settings for subsequent scans of IT8 with printed target.
Thanks, but Vuescan has no RAW output it only has TIFF or TIFF DNG, which to use? also in the Vuescan color tab just leave it at Neutral? anyway these instructions are outdated as the software have updated, I'd assume there would be more support in here since we buy the software.